Topic 2

Cell Division

Cell division is the process by which one parent cell divides to produce daughter cells. It underlies growth, tissue repair, and reproduction.

A. The Cell Cycle

What is the Cell Cycle?

  • The cell cycle is the series of events a cell goes through from its formation to its division into two daughter cells
  • It has two main phases: Interphase (preparation) and Mitotic phase / M-phase (division)
  • Interphase is the longest phase of the cell cycle — the cell grows and prepares for division
  • Interphase is sometimes incorrectly called a resting phase — in reality it is a period of intense metabolic activity
Cell Cycle Cell Cycle = Interphase + Mitotic Phase (M-phase) Interphase is the longest phase. M-phase includes mitosis + cytokinesis.

Phases of Interphase

Sub-phaseFull NameKey Events
G₁ First Growth Phase Cell grows in size; proteins and organelles are synthesised; cell carries out normal metabolic activities
S Synthesis Phase DNA replication occurs — DNA amount doubles so each daughter cell receives a complete set of chromosomes
G₂ Second Growth Phase Cell continues to grow; organelles duplicate; cell prepares enzymes and proteins needed for division
⚡ MCQ Tip DNA replication occurs in the S phase of interphase — not during mitosis itself. After S phase, each chromosome consists of two identical sister chromatids.

B. Mitosis

Mitosis Overview

  • Produces 2 genetically identical daughter cells — same chromosome number as parent
  • Daughter cells are diploid (2n) — in humans: 46 chromosomes each
  • Occurs in somatic (body) cells — skin, muscle, liver, bone marrow
  • Purpose: growth, tissue repair, and asexual reproduction
  • One division cycle — PMAT + Cytokinesis: Prophase → Metaphase → Anaphase → Telophase → Cytokinesis
  • Prophase is the longest phase of mitosis
Mitosis Result 1 parent cell (2n) → 2 identical daughter cells (2n) No reduction in chromosome number. Genetically identical.

Phases of Mitosis — PMATC

PhaseKey Events
Prophase
(longest phase)
Chromosomes condense and become visible under the microscope; nuclear envelope breaks down; spindle fibres begin to form from centrioles
Metaphase Chromosomes line up at the cell's equator (metaphase plate); spindle fibres attach to centromeres of each chromosome
Anaphase
(shortest phase)
Sister chromatids are pulled apart to opposite poles by shortening spindle fibres; cell elongates
Telophase Nuclear envelopes reform around each set of chromosomes at the poles; chromosomes decondense; two nuclei form
Cytokinesis Cytoplasm divides — in animals: cleavage furrow pinches the cell; in plants: a cell plate forms between the two nuclei. Two daughter cells are produced.
⚡ Mnemonic Please Make A Tasty Cake — Prophase, Metaphase, Anaphase, Telophase, Cytokinesis

Significance of Mitosis

  • Growth: Increase in number of cells in multicellular organisms — body grows from a single fertilised egg by repeated mitosis
  • Repair & Replacement: Worn-out or damaged cells are replaced — e.g. skin cells, red blood cells
  • Asexual Reproduction: Unicellular organisms such as Amoeba reproduce by mitosis (binary fission)
  • Genetic Stability: Daughter cells are genetically identical to parent — maintains chromosome number across generations of cells

C. Meiosis

Meiosis Overview

  • Produces 4 genetically different daughter cells — half the chromosome number of the parent
  • Daughter cells are haploid (n) — in humans: 23 chromosomes each (gametes)
  • Occurs in reproductive organs (gonads) — ovaries and testes
  • Purpose: formation of gametes (sperm and egg cells) for sexual reproduction
  • Consists of two successive divisions: Meiosis I (reduction division) + Meiosis II (equatorial division)
  • Crossing over occurs during Prophase I — homologous chromosomes exchange genetic segments, creating genetic variation
Meiosis Result 1 parent cell (2n) → 4 unique daughter cells (n) Chromosome number halved. Each cell is genetically unique.
⚡ MCQ Tip Human body cells: 46 chromosomes (2n). After meiosis → gametes with 23 chromosomes (n). Fertilisation restores 46.

Meiosis I — Reduction Division

Meiosis I separates homologous chromosomes. Chromosome number is reduced from diploid (2n) to haploid (n).

PhaseKey Events
Prophase I
(longest & most complex)
Chromosomes condense; homologous chromosomes pair up (synapsis) to form bivalents (tetrads); crossing over occurs — homologs exchange DNA segments creating genetic variation; nuclear envelope breaks down
Metaphase I Bivalents (pairs of homologous chromosomes) line up at the equator; spindle fibres attach to centromeres
Anaphase I Homologous chromosomes (not sister chromatids) separate and move to opposite poles — this is the reduction step
Telophase I Two haploid nuclei form; nuclear envelopes may reform; cytokinesis may occur — two haploid cells result, each with duplicated chromosomes
⚡ Key Distinction In Anaphase I → homologous chromosomes separate. In Anaphase II (and Anaphase of mitosis) → sister chromatids separate.

Meiosis II — Equatorial Division

Meiosis II is similar to mitosis — it separates sister chromatids. No further chromosome reduction occurs.

PhaseKey Events
Prophase IIChromosomes recondense; nuclear envelope breaks down; spindle fibres form
Metaphase IIChromosomes (single, not in pairs) line up at the equator in each cell
Anaphase IISister chromatids are pulled apart to opposite poles by spindle fibres
Telophase II + CytokinesisFour haploid nuclei form; cytokinesis produces 4 haploid daughter cells — each genetically unique

Significance of Meiosis

  • Gamete Formation: Produces haploid gametes (sperm and eggs) needed for sexual reproduction
  • Maintains Chromosome Number: Halving chromosome number in gametes ensures that fertilisation restores the diploid number — e.g. 23 + 23 = 46 in humans
  • Genetic Variation: Crossing over during Prophase I shuffles genes between homologous chromosomes, producing offspring that are not genetically identical to either parent — raw material for evolution
  • Evolution: Genetic variation produced by meiosis and fertilisation drives natural selection and evolution

D. Mitosis vs Meiosis

Comparison Table

FeatureMitosisMeiosis
Daughter cells produced24
Chromosome numberDiploid (2n) — same as parentHaploid (n) — half of parent
Genetic variationNone — cells are identicalYes — crossing over and random assortment
Number of divisionsOneTwo (Meiosis I + II)
Where it occursSomatic (body) cellsGonads (ovaries & testes)
PurposeGrowth, repair, asexual reproductionSexual reproduction (gamete formation)
Crossing overDoes not occurOccurs in Prophase I
Synapsis of homologsDoes not occurOccurs in Prophase I
What separates in AnaphaseSister chromatidsAnaphase I: homologs; Anaphase II: sister chromatids
Example (humans)Skin, liver, blood cellsSperm and egg cells

Quick MCQ Revision

FactAnswer
The two main phases of the cell cycleInterphase + Mitotic (M) phase
Longest phase of the cell cycleInterphase
DNA replication occurs in which sub-phase?S (Synthesis) phase of Interphase
G₁ phase functionCell growth and protein synthesis
G₂ phase functionFurther growth; preparation of enzymes for division
Mitosis produces2 identical diploid (2n) cells
Meiosis produces4 unique haploid (n) cells
Longest phase of mitosisProphase
Mitosis phases (mnemonic)PMATC — "Please Make A Tasty Cake"
Chromosomes align at the equator (metaphase plate)Metaphase
Sister chromatids pulled to opposite polesAnaphase
Cytokinesis in plant cellsCell plate forms between two daughter nuclei
Cytokinesis in animal cellsCleavage furrow pinches the cytoplasm
Crossing over occurs inProphase I of Meiosis I — creates genetic variation
Synapsis / bivalent (tetrad) formation occurs inProphase I
Meiosis I separatesHomologous chromosomes (reduction division)
Meiosis II separatesSister chromatids (equatorial division)
Human body cells46 chromosomes (23 pairs) — diploid (2n)
Human gametes23 chromosomes — haploid (n)
Mitosis locationSomatic (body) cells — growth and repair
Meiosis locationGonads — ovaries and testes
Significance of meiosis for evolutionCrossing over produces genetic variation — raw material for natural selection
Key